Rays remain underrepresented in genomic research, with only eight high-quality reference genomes available to date. This is particularly true for stingarees (Urolophidae), a culturally significant group of rays largely endemic to Australia that are frequently encountered as fisheries bycatch. To address this gap, we generated a chromosome-level genome of the sparsely spotted stingaree (Urolophus paucimaculatus), the first reference genome for the family Urolophidae. This reference genome provides a basis for investigating ecological questions relating to dispersal, habitat use, and population connectivity in stingarees. It also creates opportunities for investigating the genetic basis of distinctive traits, including aplacental viviparity with histotrophy and venomous spines. Furthermore, the assembly will enable the development of genetic biomarkers for sex identification, species discrimination, population assignment, and provenance testing, supporting fisheries monitoring and management. As the first chromosome-level genome for a stingaree, this resource fills a major genomic gap for batoids and will facilitate comparative studies across related ray lineages, contributing to a more complete understanding of genome evolution across Chondrichthyes.