Mangroves have the capacity to export dissolved organic matter laterally into estuarine systems. In this study we investigated the dissolved organic carbon (DOC) and nutrient outwelling dynamics of a temperate estuarine mangrove system on the Hunter River, NSW. We also investigated the bioavailability of this outwelled mangrove sourced material and traced its potential movement into the estuarine food web through stable isotope analysis of zooplankton. We sampled DOC, nutrients, bioavailability (DOC and DO consumption) across the estuarine gradient and tidal fluxes in March, April, May, June and July of 2026 to compare the mangrove influenced sites to more mesohaline and marine influenced areas. Zooplankton and various source material including mangrove leaves were sampled for stable isotope analysis from these areas (δ15N, δ¹³C and δ³⁴S). We found evidence of bioavailable mangrove associated DOC being tidally outwelled within the Hunter River. Tidal flux was also found to influence DOC concentrations within mangrove systems, with DOC concentration highest in the middle of a receding tide. Mangroves sites exported NOx whilst NH4 declined over a tidal cycle. Stable isotope analysis of zooplankton revealed significant differences across the estuarine gradient, with a pronounced difference in signatures from the mangrove creek site at low tide suggesting a different basal resources for zooplankton and these were different to mangrove leaf isotope signatures. This study indicates bioavailable mangrove DOC can be outwelling in the Hunter River while showing the source-sink nutrient dynamics of mangroves. This study also displays the spatial variability in the basal source of zooplankton diets within mangrove ecosystems and estuaries more broadly