Poster Presentation Australian Society for Fish Biology & Australian Freshwater Sciences Society Conference 2026

From Research to Routine Monitoring: OSS's Omics Strategy for Freshwater Ecosystem Assessment (#58)

Andrew Harford 1 , Thomas Mooney 1 , Lisa Chandler 1 , Chris Humphrey 1 , Anthony Chariton 2
  1. Department of Climate Change, Energy, the Environment and Water, Eaton, NT, Australia
  2. School of Natural Sciences, Macquarie University, Sydeny, NSW, Australia

The Office of the Supervising Scientist (OSS) is responsible for protecting aquatic ecosystems in the Alligator Rivers Region and supporting the rehabilitation and closure of the Ranger uranium mine in Kakadu National Park. To meet the need for increasingly comprehensive, efficient and defensible environmental monitoring, OSS has developed a long-term omics strategy focused on integrating DNA-based technologies into freshwater ecosystem assessment. The strategy builds on more than a decade of genomics-related research and aims to transition omics tools from experimental applications to routine regulatory monitoring.

Current activities include the development of DNA barcode reference libraries, environmental DNA (eDNA) and metabarcoding approaches for aquatic macroinvertebrates and vertebrates, bioinformatic pipelines for processing large genomic datasets, and quality assurance frameworks to support regulatory acceptance. OSS is also investigating the use of genomics for terrestrial ecosystems and broader multi-omics approaches to improve understanding of ecosystem function and rehabilitation trajectories. These tools are designed to complement, rather than replace, traditional biomonitoring programs by increasing taxonomic resolution, reducing reliance on specialist taxonomy, improving survey efficiency and enabling assessment of ecosystem components that are difficult to monitor using conventional methods.

Looking forward, the strategy aims to establish robust standards, protocols and governance arrangements that will support the routine application of genomic monitoring in freshwater ecosystems. Future priorities include expanding species coverage, improving ecological inference from DNA data, strengthening quality assurance systems, and developing nationally consistent approaches that can be adopted by regulators and monitoring agencies. By combining traditional ecological monitoring with emerging omics technologies, OSS seeks to deliver a more holistic understanding of ecosystem condition and resilience, supporting evidence-based management of freshwater environments in northern Australia and beyond.