Increasing anthropogenic pressures on coastal ecosystems are threatening shark populations and their keystone ecological role. Multiple paternity, where a single litter is sired by multiple males, may enhance reproductive success in sharks by increasing genetic diversity and fitness, particularly in changing environmental conditions; however, population declines reduce these benefits. Although multiple paternity is thought to be common among elasmobranchs, its occurrence among sharks (Selachii) remains scattered and largely unknown in Australian waters. This study uses single nucleotide polymorphisms (SNPs) to assess the prevalence of multiple paternity among shark species in Australian waters and to determine whether the degree of multiple paternity varies among species and litters. To address this, DNA was extracted, and relatedness and kinship analyses was conducted using dartR and EMIBD9 on embryos (n=115) across 25 litters and mothers of 5 shark species from two different ecological zones, caught in the Queensland Shark Control Program between 2021 and 2026. By investigating the prevalence of multiple paternity, these findings will establish a genetic baseline to inform shark conservation management and guide future policy decisions.