Seafood is one of the world’s most widely traded food commodities, and growing awareness of illegal fisheries, mislabelling and species substitution is increasing demand for transparency across global supply chains. These challenges are particularly evident in shark fisheries and conservation, with shark products often marketed under broad or ambiguous common names that obscure species identity. Reliable species identification typically relies on laboratory based molecular methods, but these limit the ability to perform rapid, in situ verification from harvest and processing through to import and retail. We developed and optimised Loop-mediated Isothermal Amplification (LAMP) assays for the presumptive identification of gummy, school and other deep-sea sharks of fisheries and conservation interest, including species recently added to CITES regulation. Family-, genus- and species-specific primers and reaction conditions were evaluated to optimise rapid and selective detection of target shark DNA while minimising non-target amplification. Assays were evaluated for specificity, sensitivity and time to detection using verified target and non-target tissue samples. These assays provide a basis for needed field-deployable screening tools capable of producing results within 20-30 minutes. Applied at points of inspection, LAMP has the potential to strengthen fisheries monitoring, regulatory compliance, and support conservation of key shark species.